Edvotek Water Quality Testing III Experiment Kit: Multiplex PCR Testing of Water Contaminants

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

• Group size: for 25 students
• Time required: extraction - 60 minutes, PCR setup - 10 minutes, PCR - 2 hours, electrophoresis - 60 minutes, staining - 5 mins or overnight
• Kit includes: instructions, PCR EdvoBeads™, universal DNA buffer, LyphoPrimer™ mix, EdvoQuick™ DNA ladder, LyphoControl™ (complete PCR control), TE buffer, proteinase K, BactoBeads™, UltraSpec-Agarose™, electrophoresis buffer (50X), SYBR® safe stain, FlashBlue™ stain, microcentrifuge, and PCR tubes
• Storage: some components require refrigerator or freezer storage upon receipt